Generator

Part:BBa_K223040:Design

Designed by: Suzanne Bartram   Group: iGEM09_Stanford   (2009-10-17)


SoxR Gene: Produces the SoxR protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 486
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 487
    Illegal PstI site found at 501
    Illegal NotI site found at 7
    Illegal NotI site found at 494
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 487
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 487
    Illegal PstI site found at 501
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Can be put on both a high, pSB1A3 with AmpR, and low copy plasmid.


Source

GenBank Escherichia coli E24377A

http://www.ncbi.nlm.nih.gov/nuccore/NC_009801.1/from=4616553&to=4617017&report=fasta

References